Buffers_Reagents

Protein Array Reagents

Protein Arraying Reagents Optimized for Use on FAST Slides

Protein Array Buffer - Protein Array Wash Buffer - Protein Array Blocking Buffer

Protein Arraying Buffer
Schleicher & Schuell BioScience has used its immunodiagnostic experience to develop a Protein Arraying Buffer that enhances protein stability and signal intensity. This proprietary buffer is highly recommended for research and product development where sensitivity of detection and long-term stability of protein arrays are important. This is the same arraying buffer used in the FAST^® Pak kits.

S&S Protein Arraying Reagents
Protein Array Buffer Influence of Buffer on Antibody Array Shelf-Life A monoclonal antibody to creatine kinase (0.1 mg/ml) was arrayed in either PBS or Protein Arraying Buffer using split pins. Cy ™ 5 labeled creatine kinase in solution was detected at the indicated time points. The graph demonstrates the increased stability of the Protein Arraying Buffer through 771 days.

Ordring Information - Array Buffers & Reagents

Description	Qty/Pkg	Item #
Protein Arraying Buffer (2x)	4 x 10 ml	10 485 331
Protein Array Wash Buffer (10x)	4 x 125 ml	10 485 330
Protein Array Blocking Buffer (1x)	1 x 100 ml	10 485 356

To order contact Customer Service at: 1-800-245-4024 or 603-352-3810

Increased Stability with Superior Signal

S&S Protein Arraying Reagents
Protein Array Blocking Buffer Superior Blocking Capabilities Reduction of non-specific protein-protein interaction. Monoclonal capture antibodies were arrayed on FAST Slides and probed with an antibody cocktail consisting of 16 biotinylated polyclonal antibodies followed by Streptavidin-Cy ™ 5 detection. Images were taken at identical laser/PMT setting. The first row of each array field (3 columns x 4 rows) is a positive detection control. TOP: Unspecific binding of biotinylated antibodies using standard TBS TWEEN 20, 0.1%. BOTTOM: Reduction of unspecific antibody — antibody interactions by the Protein Array Blocking Buffer.
Blocking with TBS Tween 20	Blocking with Protein Array Blocking Buffer

Protein Array Wash Buffer
The S&S Protein Array Wash Buffer has been optimized for efficient washing of protein microarrays to ensure optimum results. Conveniently supplied as 10x concentrate, this buffer is used as the washing buffer in the FAST Pak kits, and FAST Quant

Protein Array Blocking Buffer
The proprietary Protein Array Blocking Buffer has been specifically developed and formulated for fluorescent detection systems. The Protein Array Blocking Buffer is used as blocking solution in the FAST^® Quant Kit and all FAST Pak kits. Features:

Highly efficient blocking of protein microarrays
Strong reduction of non-specific antibody-antibody interactions
Minimal effects on specific antibody-antigen interactions
Superior signal-to-noise ratios in protein microarray applications

Protein Array
Buffers & Reagents

S&S arraying reagents provide the quality and reliability you need for enhanced:

Long-term stability of proteins

Signal intensity

Reduction of non-specific antibody-antibody interactions

FAST PAK Kit Protocol (PDF)
FAST Slides - Arraying of Proteins (PDF)

FAST Slides
FAST Frame
FAST Pak
FAST Quant
MicroCASter
Incubation Chambers

Site Map

Protein Array Blocking Buffer The proprietary Protein Array Blocking Buffer has been specifically developed and formulated for fluorescent detection systems. The Protein Array Blocking Buffer is used as blocking solution in the FAST® Quant Kit and all FAST Pak kits. Features:

Strong reduction of non-specific antibody-antibody interactions

Minimal effects on specific antibody-antigen interactions

Superior signal-to-noise ratios in protein microarray applications

Protein Array Blocking Buffer
The proprietary Protein Array Blocking Buffer has been specifically developed and formulated for fluorescent detection systems. The Protein Array Blocking Buffer is used as blocking solution in the FAST^® Quant Kit and all FAST Pak kits. Features: